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Abstract

Lyse red blood cells while leaving the Plasmodium falciparum parasite intact with it''s parasite membrane and parasitophorous vacoule membrane. Typically used right before freezing down parasites for genomic DNA extraction, or for getting rid of hemoglobin right before running a Western Blot on parasite extracts.

Reagents

0.15% Saponin in PBS
1X PBS
12 mL of Plasmodium falciparum blood culture at 4% hematocrit

Equipment

Refrigerated centrifuge capable of holding 15 mL conical tubes
Microcentrifuge

Procedure

Centrifuge parasite culture at 1400 rpm （~484xg） for 3 minutes.
Aspirate media.
Resuspend pellet in 1 ml of 0.15% Saponin （in aliquots in freezer） and transfer to a 1.5 ml eppendorf tube.
Incubate for 5 minutes on ice and vortex each minute.
Spin at 6000 rpm for 3 minutes at 4 degrees C.
Wash with 1 ml 1X PBS （spin at 6000 rpm for 3 minutes） 3 to 4 times.
1. Each wash step consists of aspirating the supernatant and resuspending the pellet with new wash buffer, before centrifuging.
Aspirate off last wash.
Store at -20 degrees C until ready to use.

xy-E12565【中文名称】：大鼠P选择素(P-Selectin/CD62P)ELISA试剂盒【英文名称】：Rat P-selectin (P-Selectin/CD62P) ELISA kit规格：96T/48T

xy-E12566【中文名称】：大鼠抗α-胞衬蛋白抗体IgG/IgA(α-Fodrin IgG/IgA)ELISA试剂盒【英文名称】：Rat anti- α- fodrin antibody IgG / IgA (α-Fodrin IgG / IgA) ELISA Kit规格：96T/48T

xy-E12567【中文名称】：大鼠脂蛋白α(Lp-α)ELISA试剂盒【英文名称】：Lipoprotein rat α (Lp-α) ELISA kit规格：96T/48T

xy-E12568【中文名称】：大鼠凝血因子Ⅷ相关抗原(FⅧ-Ag)ELISA试剂盒【英文名称】：Rat coagulation factor Ⅷ related antigen (F Ⅷ-Ag) ELISA kit规格：96T/48T

xy-E12569【中文名称】：大鼠凝血因子IX(FIX)ELISA试剂盒【英文名称】：Blood Coagulation Factor IX (FIX) ELISA Kit规格：96T/48T

xy-E12570【中文名称】：大鼠凝血因子Ⅹ(FⅩ)ELISA试剂盒【英文名称】：Blood Coagulation Factor Ⅹ (F Ⅹ) ELISA Kit规格：96T/48T

xy-E12571【中文名称】：大鼠血管性血友病因子/瑞斯托霉素辅因子(VWF)ELISA试剂盒【英文名称】：Rat von Willebrand factor / ristocetin cofactor (VWF) ELISA kit规格：96T/48T

xy-E12572【中文名称】：大鼠纤溶酶抗纤溶酶复合物(PAP)ELISA试剂盒【英文名称】：Rats plasmin -antiplasmin complexes (PAP) ELISA Kit规格：96T/48T

xy-E12573【中文名称】：大鼠凝血酶抗凝血酶复合物(TAT)ELISA试剂盒【英文名称】：Rat thrombin -antithrombin complex (TAT) ELISA Kit规格：96T/48T

xy-E12574【中文名称】：大鼠抗凝血酶受体(ATR)ELISA试剂盒【英文名称】：Anti-rat thrombin receptor (ATR) ELISA kit规格：96T/48T

xy-E12575【中文名称】：大鼠凝血酶受体(TR)ELISA试剂盒【英文名称】：Rat thrombin receptor (TR) ELISA kit规格：96T/48T

xy-E12576【中文名称】：大鼠心肌肌钙蛋白Ⅰ(cTn-Ⅰ)ELISA试剂盒【英文名称】：Rat cardiac troponin Ⅰ (cTn-Ⅰ) ELISA Kit规格：96T/48T

xy-E12577【中文名称】：大鼠磷酸肌醇3激酶(PI3K)ELISA试剂盒【英文名称】：Inositol 3-kinase phosphorylation in rat (PI3K) ELISA kit规格：96T/48T