抗生长激素抗体(Anti-GHAb)检测试剂盒

ELISA Kit for Anti-Growth Hormone Antibody (Anti-GHAb)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Specificity

This assay has high sensitivity and excellent specificity for detection of Anti-Growth Hormone Antibody (Anti-GHAb).
No significant cross-reactivity or interference between Anti-Growth Hormone Antibody (Anti-GHAb) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Anti-Growth Hormone Antibody (Anti-GHAb) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Growth Hormone Antibody (Anti-GHAb) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	79-101	82
EDTA plasma(n=5)	86-98	95
heparin plasma(n=5)	95-105	101

Precision

Intra-assay Precision (Precision within an assay):抗生长激素抗体(Anti-GHAb)检测试剂盒 3 samples with low, middle and high level Anti-Growth Hormone Antibody (Anti-GHAb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Growth Hormone Antibody (Anti-GHAb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Growth Hormone Antibody (Anti-GHAb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	92-101%	80-97%	79-88%	89-96%
EDTA plasma(n=5)	78-104%	81-102%	99-105%	98-105%
heparin plasma(n=5)	83-105%	95-103%	81-95%	80-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, 抗生长激素抗体(Anti-GHAb)检测试剂盒operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37^oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37^oC;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37^oC;
6. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to 抗生长激素抗体(Anti-GHAb)检测试剂盒the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Growth Hormone Antibody (Anti-GHAb) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Growth Hormone Antibody (Anti-GHAb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.